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A PCR-BASED DIAGNOSIS
OF TUBERCULOSIS FROM MENSTRUAL BLOOD OF INFERTILE WOMEN
S. Pall1, R. Goswami2 , M. Das2, A. Paull1, B.Paul1, C.S.
Chakrabarti2
1
Human Genetic Engineering Research Centre,
11 Gangapuri, Purba Putiary , Kolkata- 93.
E-mail: gerc@vsnl.net
2Cytogenetics
Laboratory, Department of Zoology,
The University of Burdwan, Burdwan- 713104.
E-mail: zoocsc_bu@indiatimes.com
A
very fine and less expensive technique has been developed
in our laboratory for the identification of Mycobacterium
tuberculosis from the patients reported as infertile.
A large number of patients referred as infertile came
to our laboratory for the detection of genetic problems.
While studying these patients we noticed that many of
them suffered from genital/ tuberculosis. This was detected
by Polymerase Chain Reaction (PCR) technique using menstrual
blood as the sample instead of conventional invasive techniques.
Majority of the patients were cured after therapeutic
intervention. Out of them some women conceived normally
and even gave live births. We hope that this new, non-invasive
technique will immensely benefit the society specially
the people suffering from tuberculosis-related infertility.
Abstracts
of the 20th Annual Meeting of the European Society of
Human Reproduction and Embryology
Berlin
Germany
27 – 30 June 2004
O-100
Subclinical genital tuhetculusis in repeated IVF failure
P,
Purvita Dam. S, K, S.K. Goswami, H.H. Shirazee S, Ghosh,
R.
Chattopadhyay, S.C. Pal, B.N. Chakravarty.
Institute
of Reproductive Medicine, IVF Unit Calcutta, India 2 Human
Gegetic Engineering Rearch Center, Genetic Unit, Calcutta,
India. Introduction:
genital tuberculosis is often a silent discase, present
for years without producing any symptom. Implantatuion
failure following IVF is unexplained infertility may be
due to latent genital tuberculosis possibly due to impaired
subendometrical blood flow (SEBF). The objective of this
study was to establish whether or not impaired SEBF .
The objective of this study was to establish whether or
not impaired SEBF in subelinical genital tuberculosis
in appearently unexplained infertility could be a cause
of repeated IVF failure. Material
and methods: A total of 81 patients (aged 25-37 yrs.)
of unexplained infertility, between Sep 2002 to Aug 2003,
infertility of 3-10 yrs, having repeated IVF failure (>
3 attempts) were subjected to PCR against Mycobacterium
Tuberculosis (Mye Tub) from menstrual blood/endometrial
tissue. IS 6110 probe against Myc Tub DNA was used and
acid fast bacilli staining of slides and BACTEC culture
form the same sample performed. Patients who at least
had PCR positive (63 patients, 77.77%) were given ATD
for three months and subjected to repeat PCR. Once negative,
attempt for pregnancy was made through repeat IVE, either
fresh IVF-ET or FET. Patients remaining positive were
put on continued ATD for another 3 months and PCR repeated
before subjecting them to IVE 5 patients with drug resistant
tuberculosis were referred for further management. Out
of 58 patients, 40 underwent fresh pick up and transfer
and 18 had FET. Fresh cycles (cycle A1) were compared
with previous attempts (Cycle A2) and evaluated on the
basis of amount of gonadotropin required, terminal E2,
number of oocytes retrieved, fertilization rate, embroyo
quality, endomencies. Frozen cycles (Cycle B1) were compared
with previous attempts (Cycle B2) and evaluated on the
baiss endomettrial thickness SEBF, pregnancy rates and
orgoing pregnancies. Result:
Amount of gonadotropin required were 2449 + 854 Vs 2797
+ 73710, terminal E2 1805 + 1023 Vs 1717 + 920 pg/ml,
oocytes retrieved 9.35 + 4.14 Vs. 3.8 + 1.6, in cycle
A1 and A2 respectively. Endometrial thickness was 11.3
+ 1.5 Vs 8.9 + 1.3 mm (p<0.05) and peak systolic blood
flow (Vmax) 20.4 + 3.1 Vs 14.1 + 2.1 cms/sec (p<0.01)
in cycle A1 and A2 respectively. Clinical and orgoing
pregnancies were 20% and 15% in A1. FET cycles showed
endometrial thickness of 10.9 + 1.5 Vs. 8.6 + 1.1 mm (p<0.05)
SEBF (Vmax )19.2 + 2.9 Vs 13.6 + 2.1 cms/sec (p<0.01)
in cycle B1 and B2 respectively. In cycle B1 both clinical
and ongoing pregnan pregnancies were 11%.
Conclusion:
From this study it appears that impaired‘ SEBF due
to latent genital tuberculosis in apparently unexplained
infertility could be a latent genital tuberculosis in
apparently unexplained infertility could be a cause of
repeated IVF failure especially in the context of the
subcontinent as region prone to tuberculisis in any form.
C15
Trisomy 21 : effect of induced abortion
RUPALI PAL1, ANIRUDDHA PAL1, BODHISATTA PAL1, MITHUN DAS2,
RIDDHI GOSWAMI2, SUSIL PAL1 AND CHANDRASEKHAR CHAKRABARTI2
1 Human genetic Engineering Research Center,
11 Gangapuri, Purba Putiary, Kolkata 700 093
E-mail : hgerc@yahoo.co.in
2 Cytogenetics Laboratory, Department of Zoology,
The University of Burdwan. Burdwan 713 104
E mail : zoocsc_bu@indiatimes.com
Down
syndrome happens due to either trisomy of 21st chromosome
or translocation. It has been reported earlier that advanced
maternal age increases the chance of birth of a Down child.
In our studies we found that apart from high maternal
age, M.T.P. (Medical Termination of Pregnancy) or abortion
is also a major factor responsible for tersomy 21. Our
data suggest that abortion has a considerable effect on
the prevalence of trisomy 21. Previous studies suggest
that abortion causes imbalance in the rate of synthesis
and secretion of female reproductive hormones. These changes
may alter the conditions necessary for the development
of the normal child as a next issue, which may further
affect the normal functions of some genes located on chromosome21.
These altered genes located on chromosome21. These altered
genes located on chromosome 21. These altered genes possibly
interfere with the functioning of tubulin responsible
for normal cell division, thereby causing nondisjunction
leading to trisomic condition. We conclude that women
with history of abortion coupled with high maternal age
more susceptible to give birth to trisomy 21 babies.
Direction of Genital Tuberculosis from Menstrual Blood
of Women Suffering from Infertility: A Molecular Diagnosis
Mithun Das1, R.Goswami1, A Pal1, B.Pal1, C.S.Chakraborty2,
P.Goswami3, B.Ghosh3, J.Bhattacharya4, M.Chakraborty3,
B.N.Chakraborty3 and S.C.Pal1, 3
1 Human genetic Engineering Research Center, Kolkata -
700093
2Cytogenetics Laboratory, Department of Zoology, The University
of Burdwan, Burdwan - 713104
3Institute of Reproductive Medicine, Kolkata-700106
4IVF & Infertility Rearch Center, Kolkata - 700 068
5Corresponding Author, Human Genetic Engineering Rearch
Center, Kolkata - 700 093, India
A
new non-invasive technique has been developed for the
identification of Mycobacterium tuberculosis from the
patients reported as infertile. A large number of patients
referred as infertile were diagnosed in a different way.
We noticed that many of them were suffering from genital
tuberculosis (GUTB). This was detected through Polymerase
Chain Reaction (PCR) technique using menstrual blood as
the sample instead of other conventional invasive techniques.
Majority of the patients were cured after proper medication.
It seems that a subset women suffering from infertility
are actually suffering from GUTB. After therapeutic intervention
it was noticed that some women not only conceived normally
but gave live births as well. Therefore, we hope that
this new and non-invasive technique will be of valuable
importance in our society, especially for the people who
are suffering from tuberculosis-related infertility.
Effect of Age and Fecundity
Period on Fertility
Gargi Roy1, R chakraborty1, B.Pal1, S.K.Goswami2, P.Dam2,
S.Ghosh2, B.N.Chakravarty2 and S.C.Pal1.
1 Human Genetic Engineering Rearch center, Kolkata - 700
093, India
2 Institute of Reproductive medicine, Kolkata - 700 106,
India
Age, a vital biological factor, directly affects the fertility
of a women which is completely dependent on the fecundity
period. Fecundity, an important biological factor, is
the ability of a couple to produce a live child. For a
women, the pattern of fecundity period is completely inherited
from her maternal side. Fecundity rearches its peak at
about age 25 in both males and females. The fecundity
of women declines after 30 steadily and most rapidly after
the age of 40. Thus, higher the age groups of women, higher
the chance of infertility. But a coherent study of fecundity
period, which varies from individual to invidual, gives
a picture regarding the potency of conception. The achievement
of normal pregnancy is a most stringent test of the anatomic
and functional integrity of the reproductive and endocrine
systems of both sexes. Thus, the potency of conception,
i.e. fecundity period, ovulation and hormonal potential
indicate the span of fertility. The fecundity period,
which is of meternal inheritance, is studied at least
three generations from the maternal side. It has been
found that the fecundity period varies up to 48 years
of age in 30% of great grandmother generation, up to 44
yrs. of age among 40% of that generation and in another
30% of great grandmother generation, up to 44 yrs. of
age among 40% of that generation and in another 30% of
great generation, the fecundity period varies up to approximately
42 yrs. of age.
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