 |
Abstracts
of the
20th Annual Meeting of the European Society of
Human Reproduction and Embryology
Berlin
Germany
27 – 30 June 2004
O-100
Subclinical genital tuhetculusis in repeated IVF failure
P,
Purvita Dam. S, K, S.K. Goswami, H.H. Shirazee S, Ghosh,
R.
Chattopadhyay, S.C. Pal, B.N. Chakravarty.
Institute
of Reproductive Medicine, IVF Unit Calcutta, India 2 Human
Gegetic Engineering Rearch Center, Genetic Unit, Calcutta, India.
Introduction:
genital
tuberculosis is often a silent discase, present for years without
producing any symptom. Implantatuion failure following IVF is unexplained
infertility may be due to latent genital tuberculosis possibly due
to impaired subendometrical blood flow (SEBF). The objective of
this study was to establish whether or not impaired SEBF . The objective
of this study was to establish whether or not impaired SEBF in subelinical
genital tuberculosis in appearently unexplained infertility could
be a cause of repeated IVF failure.
Material
and methods:
A total of 81
patients (aged 25-37 yrs.) of unexplained infertility, between Sep
2002 to Aug 2003, infertility of 3-10 yrs, having repeated IVF failure
(> 3 attempts) were subjected to PCR against Mycobacterium Tuberculosis
(Mye Tub) from menstrual blood/endometrial tissue. IS 6110 probe
against Myc Tub DNA was used and acid fast bacilli staining of slides
and BACTEC culture form the same sample performed. Patients who
at least had PCR positive (63 patients, 77.77%) were given ATD for
three months and subjected to repeat PCR. Once negative, attempt
for pregnancy was made through repeat IVE, either fresh IVF-ET or
FET. Patients remaining positive were put on continued ATD for another
3 months and PCR repeated before subjecting them to IVE 5 patients
with drug resistant tuberculosis were referred for further management.
Out of 58 patients, 40 underwent fresh pick up and transfer and
18 had FET. Fresh cycles (cycle A1) were compared with previous
attempts (Cycle A2) and evaluated on the basis of amount of gonadotropin
required, terminal E2, number of oocytes retrieved, fertilization
rate, embroyo quality, endomencies. Frozen cycles (Cycle B1) were
compared with previous attempts (Cycle B2) and evaluated on the
baiss endomettrial thickness SEBF, pregnancy rates and orgoing pregnancies.
Result:
Amount of gonadotropin required
were 2449 + 854 Vs 2797 + 73710, terminal E2 1805
+ 1023 Vs 1717
+ 920 pg/ml, oocytes retrieved 9.35 + 4.14 Vs. 3.8
+ 1.6, in cycle A1 and A2 respectively. Endometrial thickness
was 11.3 + 1.5
Vs 8.9 + 1.3
mm (p<0.05) and peak systolic blood flow (Vmax) 20.4 + 3.1
Vs 14.1 + 2.1
cms/sec (p<0.01) in cycle A1 and A2 respectively. Clinical and
orgoing pregnancies were 20% and 15% in A1. FET cycles showed endometrial
thickness of 10.9 + 1.5
Vs. 8.6 + 1.1
mm (p<0.05) SEBF (Vmax )19.2 + 2.9 Vs 13.6 + 2.1
cms/sec (p<0.01) in cycle B1 and B2 respectively. In cycle B1
both clinical and ongoing pregnan pregnancies were 11%.
Conclusion:
From this study it appears that impaired‘ SEBF due to latent genital
tuberculosis in apparently unexplained infertility could be a latent
genital tuberculosis in apparently unexplained infertility could
be a cause of repeated IVF failure especially in the context of
the subcontinent as region prone to tuberculisis in any form.
C15 Trisomy 21 : effect of induced abortion
RUPALI PAL1, ANIRUDDHA PAL1, BODHISATTA PAL1, MITHUN DAS2, RIDDHI
GOSWAMI2, SUSIL PAL1 AND CHANDRASEKHAR CHAKRABARTI2
1 Human genetic Engineering Research Center,
11 Gangapuri, Purba Putiary, Kolkata 700 093
E-mail : hgerc@yahoo.co.in
2 Cytogenetics Laboratory, Department of Zoology,
The University of Burdwan. Burdwan 713 104
E mail : zoocsc_bu@indiatimes.com
Down
syndrome happens due to either trisomy of 21st chromosome or translocation.
It has been reported earlier that advanced maternal age increases
the chance of birth of a Down child. In our studies we found that
apart from high maternal age, M.T.P. (Medical Termination of Pregnancy)
or abortion is also a major factor responsible for tersomy 21. Our
data suggest that abortion has a considerable effect on the prevalence
of trisomy 21. Previous studies suggest that abortion causes imbalance
in the rate of synthesis and secretion of female reproductive hormones.
These changes may alter the conditions necessary for the development
of the normal child as a next issue, which may further affect the
normal functions of some genes located on chromosome21. These altered
genes located on chromosome21. These altered genes located on chromosome
21. These altered genes possibly interfere with the functioning
of tubulin responsible for normal cell division, thereby causing
nondisjunction leading to trisomic condition. We conclude that women
with history of abortion coupled with high maternal age more susceptible
to give birth to trisomy 21 babies.
Direction of Genital Tuberculosis from Menstrual
Blood of Women Suffering from Infertility: A Molecular Diagnosis
Mithun Das1, R.Goswami1, A Pal1, B.Pal1, C.S.Chakraborty2, P.Goswami3,
B.Ghosh3, J.Bhattacharya4, M.Chakraborty3, B.N.Chakraborty3 and
S.C.Pal1, 3
1 Human genetic Engineering Research Center, Kolkata - 700093
2Cytogenetics Laboratory, Department of Zoology, The University
of Burdwan, Burdwan - 713104
3Institute of Reproductive Medicine, Kolkata-700106
4IVF & Infertility Rearch Center, Kolkata - 700 068
5Corresponding Author, Human Genetic Engineering Rearch Center,
Kolkata - 700 093, India
A
new non-invasive technique has been developed for the identification
of Mycobacterium tuberculosis from the patients reported as infertile.
A large number of patients referred as infertile were diagnosed
in a different way. We noticed that many of them were suffering
from genital tuberculosis (GUTB). This was detected through Polymerase
Chain Reaction (PCR) technique using menstrual blood as the sample
instead of other conventional invasive techniques. Majority of the
patients were cured after proper medication. It seems that a subset
women suffering from infertility are actually suffering from GUTB.
After therapeutic intervention it was noticed that some women not
only conceived normally but gave live births as well. Therefore,
we hope that this new and non-invasive technique will be of valuable
importance in our society, especially for the people who are suffering
from tuberculosis-related infertility.
Effect
of Age and Fecundity
Period on Fertility
Gargi Roy1, R chakraborty1, B.Pal1, S.K.Goswami2, P.Dam2, S.Ghosh2,
B.N.Chakravarty2 and S.C.Pal1.
1 Human Genetic Engineering Rearch center, Kolkata - 700 093, India
2 Institute of Reproductive medicine, Kolkata - 700 106, India
Age, a vital biological factor, directly affects the fertility of
a women which is completely dependent on the fecundity period. Fecundity,
an important biological factor, is the ability of a couple to produce
a live child. For a women, the pattern of fecundity period is completely
inherited from her maternal side. Fecundity rearches its peak at
about age 25 in both males and females. The fecundity of women declines
after 30 steadily and most rapidly after the age of 40. Thus, higher
the age groups of women, higher the chance of infertility. But a
coherent study of fecundity period, which varies from individual
to invidual, gives a picture regarding the potency of conception.
The achievement of normal pregnancy is a most stringent test of
the anatomic and functional integrity of the reproductive and endocrine
systems of both sexes. Thus, the potency of conception, i.e. fecundity
period, ovulation and hormonal potential indicate the span of fertility.
The fecundity period, which is of meternal inheritance, is studied
at least three generations from the maternal side. It has been found
that the fecundity period varies up to 48 years of age in 30% of
great grandmother generation, up to 44 yrs. of age among 40% of
that generation and in another 30% of great grandmother generation,
up to 44 yrs. of age among 40% of that generation and in another
30% of great generation, the fecundity period varies up to approximately
42 yrs. of age.
|
